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上海澤泉科技股份有限公司
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面議
1242
利用調制葉綠素熒光技術，測量野外自然水體或培養的微藻樣品的光合作用（葉綠素熒光誘導加淬滅分析、光響應曲線等），也可測量葉綠素含量，是進行野外光合作用研究的良好工具。除了測浮游植物外，可擴展探頭測量附著藻類或大型藻類。除了取水樣到樣品杯中測量外，可擴展探頭進行水下原位、連續測量，特別適合于連續監測海洋、湖泊、水庫、河流等水體的葉綠素含量以及光合活性。
主要功能
1）可測熒光誘導曲線并進行淬滅分析
2）可測光響應曲線和快速光曲線（RLC）
3）可測水樣的葉綠素a濃度
4）可測量水樣的下列光合指標活性：
* 光合效率和光合速率（相對電子傳遞速率）
* 藻類的潛在**光合效率（“生長潛能”）
* 藻類的光保護能力
* 藻類耐受強光的能力
5）51個內置模式菜單，方便參數設置和標準測量
6）系統I用于浮游植物研究，系統II用于大型藻類研究，系統III用于連續監測水體光合作用
應用領域
測量野外自然水樣或實驗室培養的微藻樣品的光合作用，三套系統可供選擇，可應用于水生生物學、水域生態學、海洋學、湖沼學等領域，檢測限達0.1 μgChl/L。可用于有害藻華的早期預警。與PHYTO-PAM的**區別在于，WATER-PAM不能進行浮游植物分類。
測量參數
Fo， Fm， Fv/Fm， F， Fm'， Fo'， Y(II)=ΔF/Fm'， qP， qN， NPQ， ETR，alpha，ETRmax， Ik， PAR和Chla含量等
主要技術參數
測量光：3個波長為650 nm的LED陣列
光化光：12個波長為660 nm的LED陣列，**連續光強2000 μmol m-2 s-1。
飽和脈沖：12個波長為660 nm的LED陣列，**閃光強度4000 μmol m-2 s-1。
信號檢測：光電倍增管檢測器（H6779-01，Hamamatsu），過載保護功能，檢測信號λ>710 nm。
數據存儲：CMOS RAM 128 KB，可存儲4000組數據。
| 系統組成 | ||
| 系統I 浮游植物版 | 系統II 附著藻類/大型藻類版 | 系統III 連續監測版 |
| 野外現場自然水體的光合作用檢測、葉綠素含量測定；室內培養的微藻樣品的生理特性研究等。 | 野外現場附著藻類（如底泥中的藻類）、大型海藻的光合活性測量；室內大型海藻生理特性研究。 | 野外現場水體光合活性監測、葉綠素含量的連續測定。 |
| 可選附件1：攪拌器，可置于系統I的上部對水樣進行攪拌，帶內置電池 | ||
| 可選附件2：球狀微型光量子探頭，可放入系統I的樣品杯中測量PAR |
部分文獻
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2.Claquin P， Longphuirt SN， Fouillaron P， Huonnic P， Ragueneau O， Klein C， Leynaert A: Effects of simulated benthic fluxes on phytoplankton dynamic and photosynthetic parameters in a mesocosm experiment (Bay of Brest， France). Estuarine， Coastal and Shelf Science2010， 86:93-101.[WATER-PAM]
3.Deblois CP， Juneau P: Relationship between photosynthetic processes and microcystin in Microcystis aeruginosa grown under different photon irradiances. Harmful Algae2010， 9:18-24.[WATER-PAM]
4.Guan W， Gao K: Impacts of UV radiation on photosynthesis and growth of the coccolithophoreEmiliania huxleyi (Haptophyceae). Environmental and Experimental Botany2010， 67:502-508.[WATER-PAM]
5.Guan W-C， Gao K-S: Enhanced calcification ameliorates the negative effects of UV radiation on photosynthesis in the calcifying phytoplankter Emiliania huxleyi Chinese Science Bulletin2010， 55(7):588-593.[WATER-PAM]
6.Helbling EW， Pérez DE， Medina CD， Lagunas MG， Villafañe VE: Phytoplankton distribution and photosynthesis dynamics in the Chubut River estuary (Patagonia， Argentina) throughout tidal cycles Limnology and Oceanography2010， 55(1):55-65.[WATER-PAM]
7.Ji C-F， Legrand J， Pruvost J， Chen Z-A， Zhang W: Characterization of hydrogen production by Platymonas Subcordiformis in torus photobioreactor International Journal of Hydrogen Energy2010:in press.[WATER-PAM]
8.Korbee N， Mata M， Figueroa F: Photoprotection mechanisms against ultraviolet radiation in Heterocapsa sp. (Dinophyceae) are influenced by nitrogen availability: Mycosporine-like amino acids vs. xanthophyll cycle Limnology and Oceanography2010， 55(2):899-908.[WATER-PAM]
9.Tanabe Y， Ohtani S， Kasamatsu N， Fukuchi M， Kudoh S: Photophysiological responses of phytobenthic communities to the strong light and UV in Antarctic shallow lakes Polar Biology2010， 33(1):85-100.[WATER-PAM]
10.Xiao Y， Liu Y， Wang G， Hao Z， An Y: Simulated microgravity alters growth and microcystin production in Microcystis aeruginosa (cyanophyta) Toxicon2010:in press.[WATER-PAM]
11.夏建榮， 田其然， 高坤山: 經濟海藻紅毛菜原位光合作用日變化. 生態學報2010， 30(6):1524-1531.[WATER-PAM]
12.Buma AGJ， Sjollema SB， van de Poll WH， Klamer HJC， Bakkerb\ JF: Impact of the antifouling agent Irgarol 1051 on marine phytoplankton species Journal of Sea Research2009， 61(3):133-139.[PAM-2000， WATER-PAM]
13.Coelho H， Calado R， Olaguer-Feliú AO， Vieira S， Queiroga H， Serôdio J: Nondestructive quantification of phytoplankton gut content of brachyuran crab megalopae using in vivo chlorophyll a fluorescence. Journal of Plankton Research2009， 31:577-581.[WATER-PAM]
14.Drath M， Baier K， Forchhammer K: An alternative methionine aminopeptidase， MAP-A， is required for nitrogen starvation and high-light acclimation in the cyanobacterium Synechocystis sp. PCC 6803. Microbiology2009， 155:1427-1439.[WATER-PAM]
15.Fredersdorf J， Müller R， Becker S， Wiencke C， Bischof K: Interactive effects of radiation， temperature and salinity on different life history stages of the Arctic kelp Alaria esculenta (Phaeophyceae) Oecologia2009， 160(3):483-492.[PAM-2100， WATER-PAM]
16.Gao Y， Xiong W， Li X-b， Gao C-F， Zhang Y-l， Li H， Wu Q-y: Identification of the proteomic changes in Synechocystis sp. PCC 6803 following prolonged UV-B irradiation. Journal of Experimental Botany2009， 60(4):1141-1154.[WATER-PAM]
17.Gradinger R: Sea-ice algae: Major contributors to primary production and algal biomass in the Chukchi and Beaufort Seas during May/June 2002. DeepSeaResearch2009， 56:1201-1212.[WATER-PAM]
18.Harada H， Vila-Costa M， Cebrian J， Kiene RP: Effects of UV radiation and nitrate limitation on the production of biogenic sulfur compounds by marine phytoplankton Aquatic Botany2009， 90(1):37-42.[WATER-PAM]
19.Kromkamp JC， Beardall J， Sukenik A， Kopeck J， Masojidek J， van Bergeijk S， Gabai S， Shaham E， Yamshon A: Short-term variations in photosynthetic parameters of Nannochloropsis cultures grown in two types of outdoor mass cultivation systems. Aquatic Microbial Ecology2009， 56:309-322.[DUAL-PAM， Flow-Through WATER-PAM]
20.Kunz H-H， Scharnewski M， Feussner K， Feussner I， Flügge U-I， Fulda M， Gierth M: The ABC Transporter PXA1 and Peroxisomal -Oxidation Are Vital for Metabolism in Mature Leaves of Arabidopsis during Extended Darkness The Plant Cell2009， 21:2733-2749.[IMAGING-PAM， WATER-PAM]
21.Mackey KRM， Rivlin T， Grossman AR， Post AF， Paytan A: Picophytoplankton responses to changing nutrient and light regimes during a bloom. Marine Biology2009， 156(8):1531-1546.[WATER-PAM]
22.Manes SS， Gradinger R: Small scale vertical gradients of Arctic ice algal photophysiological properties. Photosynthesis Research2009， 102(1):53-66.[WATER-PAM]
23.Obata M， Toda T， Taguchi S: Using chlorophyll fluorescence to monitor yields of microalgal production Journal of Applied Phycology2009， 21(3):315-319.[WATER-PAM]
24.Paytan A， Mackey KRM， Chen Y， Lima ID， Doney SC， Mahowald N， Labiosa R， Post AF: Toxicity of atmospheric aerosols on marine phytoplankton. Proc Natl Acad Sci USA2009， 106:4601-4605.[WATER-PAM]
25.Ran C， Zhang F， Sun H， Zhao B: Effect of culture medium on hydrogen production by sulfur-deprived marine green algae Platymonas subcordiformis Biotechnology and Bioprocess Engineering2009， 14(6):835-841.[WATER-PAM]
26.Roleda MY， Campana GL， Wiencke C， Hanelt D， Quartino ML， Wulff A: Sensitivity of antarctic Urospora penicilliformis (Ulotrichales， Chlorophyta) to ultraviolet radiation is life-stage dependent. Journal of Phycology2009， 45:600-609.[WATER-PAM]
27.Sukenik A， Beardall J， Kromkamp JC， Kopeck J， Masojídek J， van Bergeijk S， Gabai S， Shaham E， Yamshon A: Photosynthetic performance of outdoor Nannochloropsis mass cultures under a widerange of environmental conditions. Aquatic Microbial Ecology2009， 56(2-3):297-308.[DUAL-PAM-100， FLOW THROUGH WATER-PAM]
28.van de Poll WH， Janknegt PJ， van Leeuwe MA， Visser RJW， Buma AGJ: Excessive irradiance and antioxidant responses of an Antarctic marine diatom exposed to iron limitation and to dynamic irradiance Journal of Photochemistry and Photobiology B: Biology2009， 94(1):32-37.[WATER-PAM]
29.Wu H， Gao K， Wu H: Responses of a marine red tide alga Skeletonema costatum (Bacillariophyceae) to long-term UV radiation exposures. Journal of Photochemistry and Photobiology B: Biology2009， 94:82-86.[WATER-PAM]
30.陳書秀， 梁英: 光照強度對雨生紅球藻葉綠素熒光特性及蝦青素含量的影響. 南方水產2009， 5(1):1-8.[WATER-PAM]
31.賴永忠， 高坤山: 陽光紫外輻射對室內水培發狀念珠藻生理特性的影響. 水生生物學報2009， 33(3):435-441.[WATER-PAM]
32.梁英， 馮力霞， 田傳遠: 高溫脅迫對球等鞭金藻3011和8701葉綠素熒光特性的影響. 水產學報2009， 33(1):37-44.[WATER-PAM]
33.朱喜鋒， 鄒定輝， 簡建波， 陳偉洲， 劉慧慧， 杜虹: 龍須菜對重金屬銅脅迫的生理響應. 應用生態學報2009， 20(6):1438-1444.[WATER-PAM]
34.Bailey S， Melis A， Mackey KRM， Cardol P， Finazzi G， Dijken Gv， Berge GM， Arrigo K， Shrager J， Grossman A: Alternative photosynthetic electron flow to oxygen in marine Synechococcus Biochimica et Biophysica Acta2008， 1777(3):269-276.[DUAL-PAM-100， WATER-PAM]
35.Drath M， Kloft N， Batschauer A， Marin K， Novak J， Forchhammer K: Ammonia triggers photodamage of photosystem II in the cyanobacteriumSynechocystis sp. strain PCC 6803. Plant Physiology2008， 147:206-215.[WATER-PAM]
36.Gao K， Li P， Watanabe T， Helbling EW: Combined effects of ultraviolet radiation and temperature on morphology， photosynthesis， and DNA of Arthrospira (Spirulina) platensis (Cyanophyta). Journal of Phycology2008， 44(3):777-786.[WATER-PAM]
37.Gao K， Ma Z: Photosynthesis and growth of Arthrospira (Spirulina) platensis (Cyanophyta) in response to solar UV radiation， with special reference to its minor variant. Environmental and Experimental Botany2008， 63(1-3):123-129.[WATER-PAM]
38.Goto N， Kihira M， Ishida N: Seasonal distribution of photosynthetically active phytoplankton using pulse amplitude modulated fluorometry in the large monomictic Lake Biwa， Japan. Journal of Plankton Research2008， 30(10):1169-1177.[WATER-PAM]
39.Guan W， Gao K: Light histories influence the impacts of solar ultraviolet radiation on photosynthesis and growth in a marine diatom， Skeletonema costatum. Journal of Photochemistry and Photobiology B: Biology2008， 91(2-3):151-156.[WATER-PAM]
40.Guo Z， Chen Z， Zhang W， Yu X， Jin M: Improved hydrogen photoproduction regulated by carbonylcyanide m -chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor Biotechnology Letters2008， 30(5):877-883.[WATER-PAM]
41.Hill R， Ralph PJ: Impact of bleaching stress on the function of the oxygen evolving complex of zooxanthellae from scleractinian corals. Journal of Phycology2008， 44(2):299-310.[WATER-PAM]
42.Mojaat M， Foucault A， Pruvost J， Legrand J: Optimal selection of organic solvents for biocompatible extraction of β-carotene from Dunaliella salina Journal of Biotechnology2008， 133(4):433-441.[WATER-PAM]
43.梁英， 陳書秀， 田傳遠， 王燕: 溫度對三角褐指藻葉綠素熒光特性及生長的影響. 中國海洋大學學報2008， 38(3):377-383.[WATER-PAM]
44.梁英， 金月梅， 田傳遠: 磷限制及恢復對小球藻葉綠素熒光特性的影響. 南方水產2008， 4(4):1-7.[WATER-PAM]
45.梁英， 金月梅， 田傳遠: 氮磷濃度對綠色巴夫藻生長及葉綠素熒光參數的影響. 海洋湖沼通報2008:122-128.[WATER-PAM]
46.梁英， 王帥， 馮力霞， 田傳遠: 重金屬脅迫對纖細角毛藻生長及葉綠素熒光特性的影響. 中國海洋大學學報2008， 38(1):59-67.[WATER-PAM]
47.劉樹霞， 鄒定輝， 徐軍田， 高坤山: 不同N水平條件下羊棲菜對陽光輻射的響應. 海洋通報2008， 27(6):44-51.[WATER-PAM]
48.冉春秋， 張衛， 虞星炬， 金美芳: 硫與間氯苯腙對海水綠藻Platymonas subcordiformis光照產氫特性的影響. 太陽能學報2008， 29(8):944-949.[WATER-PAM]
49.尹翠玲， 梁英， 張秋豐: 氮濃度對球等鞭金藻3011和8701葉綠素熒光特性及生長的影響. 水產科學2008， 27(1):27-31.[WATER-PAM]
50.鄭陽， 陳兆安， 傅赟彬， 陸洪斌， 張衛: 亞心形四爿藻培養和產氫過程一體化平板光生物反應系統. 高等學校化學學報2008， 29(11):2209-2212.[WATER-PAM]
51.Ekelund NGA， Aronsson KA: Changes in chlorophyll a fluorescence in Euglena gracilis and Chlamydomonas reinhardtii after exposure to wood-ash Environmental and Experimental Botany2007， 59(1):92-98.[WATER-PAM]
52.Gao K， Guan W， Helbling EW: Effects of solar ultraviolet radiation on photosynthesis of the marine red tide alga Heterosigma akashiwo (Raphidophyceae). Journal of Photochemistry and Photobiology B: Biology2007， 86(2):140-148.[WATER-PAM]
53.Gao K， Yu H， Brown MT: Solar PAR and UV radiation affects the physiology and morphology of the cyanobacterium Anabaena sp. PCC 7120. Journal of Photochemistry and Photobiology B: Biology2007， 89(2-3):117-124.[WATER-PAM]
54.Geoffroy L， Gilbin R， Simon O， Floriani M， Adam C， Pradines C， Cournac L， Garnier-Laplace J: Effect of selenate on growth and photosynthesis of Chlamydomonas reinhardtii Aquatic Toxicology2007， 83(2):149-158.[WATER-PAM]
55.Hill R， Ralph PJ: Post-bleaching viability of expelled zooxanthellae from the scleractinian coral Pocillopora damicornis. Marine Ecology Progress Series2007， 352:137-144.[WATER-PAM]
56.Kromkamp JC， Perkins R， Dijkman N， Consalvey M， Andres M， Reid RP: Resistance to burial of cyanobacteria in stromatolites. Aquatic Microbial Ecology2007， 48(2):123-130.[WATER-PAM， DIVING-PAM]
57.Kudela RM， Ryan JP， Blakely MD， Lane JQ， Peterson TD: Linking the physiology and ecology of Cochlodinium to better understand harmful algal bloom events: A comparative approach Harmful Algae2007， 7(3):278-292.[WATER-PAM]
58.Leu E， Falk-Petersen S， Hessen DO: Ultraviolet radiation negatively affects growth but not food quality of arctic diatoms. Limnology and Oceanography2007， 52(2):787-797.[WATER-PAM]
59.McMinn A， Ryan KG， Ralph PJ， Pankowski A: Spring sea ice photosynthesis， primary productivity and biomass distribution in eastern Antarctica， 2002-2004 Marine Biology2007， 151(3):985-995.[WATER-PAM]
60.Perkins RG， Kromkam JC， Reid RP: Importance of light and oxygen for photochemical reactivation in photosynthetic stromatolite communities after natural sand burial. Marine Ecology Progress Series2007， 349:23-32.[WATER-PAM]
61.Ralph PJ， Ryan KG， Martin A， Fenton G: Melting out of sea ice causes greater photosynthetic stress in algae than freezing in. Journal of Phycology2007， 43(5):948-956.[WATER-PAM]
62.Richter P， Helbling W， Streb C， Häder D-P: PAR and UV Effects on Vertical Migration and Photosynthesis inEuglena gracilis. Photochemistry and Photobiology2007， 83(4):818-823.[WATER-PAM]
63.Roleda MY， Zacher K， Wulff A， Hanelt D， Wiencke C: Photosynthetic performance， DNA damage and repair in gametes of the endemic Antarctic brown alga Ascoseira mirabilis exposed to ultraviolet radiation. Austral Ecology2007， 32(8):917-926.[WATER-PAM]
64.Schaeffer BA， Kamykowski D， McKay L， Sinclair G， Milligan EJ: A comparison of photoresponse among ten different Karenia brevis (Dinophyceae) isolates. Journal of Phycology2007， 43(4):702-714.[WATER-PAM]
65.Schmitt-Jansen M， Altenburger R: The use of pulse-amplitude modulated (PAM) fluorescence-based methods to evaluate effects of herbicides in microalgal systems of different complexity Toxicological and Environmental Chemistry2007， 89(4):665-681.[PHYTO-PAM， WATER-PAM， MICROSCOPY-PAM]
66.Veldhuis MJW， Timmermans KR: Phytoplankton dynamics during an in situ iron enrichment experiment (EisenEx) in the Southern Ocean: a comparative study of field and bottle incubation measurements. Aquatic Microbial Ecology2007， 47(2):191-208.[WATER-PAM]
67.Villafane VE， Gao K， Li P， Li G， Walter Helbling E: Vertical mixing within the epilimnion modulates UVR-induced photoinhibition in tropical freshwater phytoplankton from southern China. Freshwater Biology2007， 52(7):1260-1270.[WATER-PAM]
68.Wulff A， Zacher K， Hanelt D， Al-Handal A， Wiencke C: UV radiation - a threat to Antarctic benthic marine diatoms? Antarctic Science2007， 20:13-20.[DIVING-PAM， WATER-PAM]
69.Zacher K， Roleda MY， Hanelt D， Wiencke C: UV effects on photosynthesis and DNA in propagules of three Antarctic seaweeds (Adenocystis utricularis， Monostroma hariotii and Porphyra endiviifolium) Planta2007， 225(6):1505-1516.[WATER-PAM]
70.關萬春， 高坤山: 陽光紫外輻射對兩種微藻類光化學效率的影響. 水生生物學報2007， 31(4):594-599.[WATER-PAM]
71.梁英， 馮力霞， 田傳遠， 王帥: 高溫脅迫對鹽藻和塔胞藻葉綠素熒光動力學的影響. 中國水產科學2007， 14(6):961-968.[WATER-PAM]
72.梁英， 尹翠玲， 江新琴， 于云芝: 硅濃度對纖細角毛藻和三角褐指藻生長及葉綠素熒光特性的影響. 海洋水產研究2007， 28(5):89-94.[WATER-PAM]
73.劉遠， 陳兆安， 陸洪斌， 劉長發， 金美芳， 郭禎， 張衛: 亞心形扁藻培養基的優化及光合特性. 過程工程學報2007， 7(6):1197-1201.[WATER-PAM]
74.尹翠玲， 梁英， 馮力霞， 曹春暉: 氮濃度對鹽生杜氏藻和纖細角毛藻葉綠素熒光特性及生長的影響. 海洋湖沼通報2007:101-110.[WATER-PAM]
75.尹翠玲， 梁英， 張秋豐: 磷濃度對球等鞭金藻3011和8701葉綠素熒光特性及生長的影響. 海洋湖沼通報2007:88-95.[WATER-PAM]
76.尹翠玲， 梁英， 張秋豐: 磷濃度對鹽生杜氏藻和纖細角毛藻葉綠素熒光特性及生長的影響. 水產科學2007， 26(3):154-159.[WATER-PAM]
77.Cosgrove J， Borowitzka M: Applying Pulse Amplitude Modulation (PAM) fluorometry to microalgae suspensions: stirring potentially impacts fluorescence. Photosynthesis Research2006， 88(3):343-350.[WATER-PAM]
78.Leu E， FÆRØVIG PJ， HESSEN DO: UV effects on stoichiometry and PUFAs of Selenastrum capricornutum and their consequences for the grazer Daphnia magna. Freshwater Biology2006， 51(12):2296-2308.[WATER-PAM]
79.Peers G， Price NM: Copper-containing plastocyanin used for electron transport by an oceanic diatom Nature2006， 441:341-344.[WATER-PAM]
80.Roleda MY， Hanelt D， Wiencke C: Exposure to ultraviolet radiation delays photosynthetic recovery in Arctic kelp zoospores. Photosynthesis Research2006， 88(3):311-322.[WATER-PAM]
81.Roleda MY， Wiencke C， Lüder2 UH: Impact of ultraviolet radiation on cell structure， UV-absorbing compounds， photosynthesis， DNA damage， and germination in zoospores of ArcticSaccorhiza dermatodea Journal of Experimental Botany2006， 57(14):3847-3856.[WATER-PAM]
82.梁英， 馮力霞， 田傳遠， 尹翠玲: 鹽脅迫對塔胞藻生長及葉綠素熒光動力學的影響. 中國海洋大學學報2006， 36(5):726-732.[WATER-PAM]
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84.冉春秋， 張衛， 虞星炬， 金美芳， 鄧麥村: 解偶聯劑CCCP對萊茵衣藻光照產氫過程的調控. 高等學校化學學報2006， 27(1):62－66.[WATER-PAM]
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